Micro-Propagation of White-top Aster, Sericocarpus rigidus, a Threatened Species from the Garry Oak Ecosystem in British Columbia

Authors

  • Brenda Frey Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, PO Box 1000, Agassiz, British Columbia V0M 1A0
  • Chaim Kempler Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, PO Box 1000, Agassiz, British Columbia V0M 1A0
  • David L. Ehret Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, PO Box 1000, Agassiz, British Columbia V0M 1A0

DOI:

https://doi.org/10.22621/cfn.v121i1.391

Keywords:

Sericocarpus rigidus, Aster curtus, White-top Aster, micro-propagation, Garry Oak ecosystem, British Columbia

Abstract

A study was undertaken to examine the feasibility of using micro-propagation techniques to regenerate White-top Aster (Sericocarpus rigidus), a threatened species native to the Garry Oak ecosystem in coastal British Columbia. Shoot multiplication from explants was compared in three different basal media formulations. The effects of varying IAA (indole-3-acetic acid) and kinetin on shoot production were also investigated using one of the three basal media. Shoot production was most successful on modified MS (Murashige and Skoog) media containing various concentrations of BAP (benzylaminopurine) and GA3 (gibberellic acid), followed by media containing IAA and kinetin at concentrations of 2 mg per litre. Root formation occurred readily on modified MS media with IAA and reduced macronutrient and micronutrient concentrations. The study showed that S. rigidus can be successfully propagated using in vitro shoot multiplication.

Downloads

Issue

Vol. 121 No. 1 (2007)

Section

Articles

License

Copyright for Canadian Field-Naturalist content is held by the Ottawa Field-Naturalists' Club, except for content published by employees of federal government departments, in which case the copyright is held by the Crown. In-copyright content available at the Biodiversity Heritage Library is available for re-use under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 (CC BY-NC-SA 4.0) licence. For usage of content at the BHL for purposes other than those allowed under this licence, contact us.

 

To request use of copyright material,  please contact our editor, Dr. Dwayne Lepitzki: editor -at- canadianfieldnaturalist -dot- ca